Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptophan + FADH2 + chloride + O2 + H+ | Pseudomonas fluorescens | - |
7-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | P95480 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tryptophan + FADH2 + chloride + O2 + H+ = 7-chloro-L-tryptophan + FAD + 2 H2O | reaction mechanism, combined quantum mechanical/molecular mechanical (QM/MM) calculations of potential energy and free energy surfaces, using the structure, PDB ID 2AR8, and employing density functional theory. First reaction step is the formation of an arenium ion (Wheland intermediate), the second reaction step is the deprotonation of the Wheland intermediate by residue E346 oxygen, at the final stage of the mechanism residues K79 and E346 are regenerated | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptophan + FADH2 + chloride + O2 + H+ | - |
Pseudomonas fluorescens | 7-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PrnA | - |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas fluorescens |
General Information | Comment | Organism |
---|---|---|
metabolism | tryptophan 7-halogenase catalyzes chlorination of free tryptophan to 7-chlorotryptophan, which is the first step in the antibiotic pyrrolnitrin biosynthesis | Pseudomonas fluorescens |